[KO Validated] N-Cadherin Rabbit mAb

時間：2020-10-29 09:57 瀏覽次數(shù)：1617次

品牌	ABclonal	產(chǎn)品類型	抗體
產(chǎn)品名稱	[KO Validated] N-Cadherin Rabbit mAb	產(chǎn)品規(guī)格	50ul /100ul /200ul
產(chǎn)品目錄號	A19083	產(chǎn)品目錄價	800/1200/2000

背景

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]

免疫原信息

免疫原	A synthesized peptide derived from human N Cadherin
序列	Email for sequence
基因ID	1000
Swiss Prot	P19022
別名	CD325;CDHN;CDw325;NCAD;N Cadherin;CDH2
研究領(lǐng)域	Cancer癌癥Invasion and Metastasis侵襲和轉(zhuǎn)移 Cell Biology & Developmental Biology細(xì)胞生物學(xué)-發(fā)育生物學(xué)Cell Cycle細(xì)胞周期Centrosome中心體 Cell Biology & Developmental Biology細(xì)胞生物學(xué)-發(fā)育生物學(xué)Cell Adhesion細(xì)胞粘附Cadherins鈣黏著蛋白 Cell Biology & Developmental Biology細(xì)胞生物學(xué)-發(fā)育生物學(xué)Cell Adhesion細(xì)胞粘附Tight Junctions緊密連接 Cell Biology & Developmental Biology細(xì)胞生物學(xué)-發(fā)育生物學(xué)Wnt/β-Catenin Signaling PathwayWnt/β-Catenin信號通路 Immunology & Inflammation免疫學(xué)與炎癥CDsCD分子 Stem Cells干細(xì)胞Hematopoietic Progenitors造血祖細(xì)胞

應(yīng)用

反應(yīng)物種	Human, Mouse, Rat
驗證應(yīng)用	WB IHC IF
推薦稀釋比&實驗步驟	WB 1:500 - 1:2000 [ 實驗步驟 ] IHC 1:50 - 1:200 [ 實驗步驟 ] IF 1:50 - 1:200 [ 實驗步驟 ]
理論分子量	140kDa
實際分子量	140kDa
存儲緩沖液	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
實驗方法	Western blotting
陽性樣本	HeLa,293T,NIH/3T3,C6
細(xì)胞定位
純化方式	Affinity purification

[KO Validated] N-Cadherin Rabbit mAb 檢測圖片

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A19083) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).

Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).

Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)

Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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[KO Validated] N-Cadherin Rabbit mAb

背景

免疫原信息

應(yīng)用

[KO Validated] N-Cadherin Rabbit mAb 檢測圖片